We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical applications of hES cells.
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H.N. acknowledges funding from the University of Michigan Rackham Predoctoral Fellowship. J.L. gratefully acknowledges support from the National Science Foundation (NSF) in the form of a CAREER grant and funding from the NSF under the MRI program. We would like to thank J.H. Elisseeff and N. Hwang for their insightful comments throughout this study. We further thank J. Garcia-Perez, M. Morell, L.S.D. Emmett, M. Dzaman, M. Bormann, C. Pacut, N.Leff and J. MacDonald for their valuable assistance. We would also like to thank C. Smith, M. Yoshida and S. Brown for their invaluable comments on the manuscript. This research was supported by US National Institutes of Health grants P20 GM-069985, R01 DE016530 and the National Institute of Dental and Craniofacial Research T32 Tissue Engineering and Regeneration Training Program DE 07057.
The authors declare no competing financial interests.
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Villa-Diaz, L., Nandivada, H., Ding, J. et al. Synthetic polymer coatings for long-term growth of human embryonic stem cells. Nat Biotechnol 28, 581–583 (2010) doi:10.1038/nbt.1631
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