Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data

Nature Biotechnology volume 27, pages51–57(2009)Cite this article

Abstract

Crystallization is the most serious bottleneck in high-throughput protein-structure determination by diffraction methods. We have used data mining of the large-scale experimental results of the Northeast Structural Genomics Consortium and experimental folding studies to characterize the biophysical properties that control protein crystallization. This analysis leads to the conclusion that crystallization propensity depends primarily on the prevalence of well-ordered surface epitopes capable of mediating interprotein interactions and is not strongly influenced by overall thermodynamic stability. We identify specific sequence features that correlate with crystallization propensity and that can be used to estimate the crystallization probability of a given construct. Analyses of entire predicted proteomes demonstrate substantial differences in the amino acid–sequence properties of human versus eubacterial proteins, which likely reflect differences in biophysical properties, including crystallization propensity. Our thermodynamic measurements do not generally support previous claims regarding correlations between sequence properties and protein stability.

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Figure 1: Protein stability does not strongly influence success in crystal-structure solution.
Figure 2: Hydrodynamic properties strongly influence success in crystal structure solution.
Figure 3: Correlations between sequence characteristics and success in crystal structure solution.
Figure 4: Four major predictors of success in crystal structure solution.
Figure 5: Performance of the PXS metric predicting probability of successful crystal-structure determination.

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Acknowledgements

This work was supported by Protein Structure Initiative grants from the National Institutes of Health (NIH) to the Northeast Structural Genomics Consortium and the Center for High-Throughput Structural Biology. The full staffs of these consortia contributed to the experimental data analyzed in this paper. W.N.P. II was supported in part by an NIH training grant to the Department of Biological Sciences at Columbia, and S.K.H. was supported in part by a National Science Foundation grant to J.F.H. The authors thank Wayne Hendrickson and Liang Tong for support and advice and John Schwanoff and the New York Structural Biology Center for maintenance of the X4 beamlines at Brookhaven National Laboratory.

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Affiliations

  1. Northeast Structural Genomics Consortium, 702A Fairchild Center, MC2434, Columbia University, New York, 10027, New York, USA

    W Nicholson Price II, Yang Chen, Samuel K Handelman, Helen Neely, Philip Manor, Richard Karlin, Rajesh Nair, Jinfeng Liu, Michael Baran, John Everett, Saichiu N Tong, Farhad Forouhar, Swarup S Swaminathan, Thomas Acton, Rong Xiao, Joseph R Luft, Angela Lauricella, George T DeTitta, Burkhard Rost, Gaetano T Montelione & John F Hunt

  2. Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, 10027, New York, USA

    W Nicholson Price II, Yang Chen, Samuel K Handelman, Helen Neely, Philip Manor, Richard Karlin, Farhad Forouhar, Swarup S Swaminathan & John F Hunt

  3. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, 10032, New York, USA

    Rajesh Nair, Jinfeng Liu & Burkhard Rost

  4. Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey, 08854, USA

    Michael Baran, John Everett, Saichiu N Tong, Thomas Acton, Rong Xiao & Gaetano T Montelione

  5. Hauptman-Woodward Institute, 700 Ellicott Street, Buffalo, 14203, New York, USA

    Joseph R Luft, Angela Lauricella & George T DeTitta

  6. Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey, 08854, USA

    Gaetano T Montelione

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Correspondence to John F Hunt.

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Figures 1–20, Tables 1–4, Methods, Notes (PDF 2442 kb)

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Price II, W., Chen, Y., Handelman, S. et al. Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data. Nat Biotechnol 27, 51–57 (2009). https://doi.org/10.1038/nbt.1514

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