By analyzing five human embryonic stem (hES) cell lines over long-term culture, we identified a recurrent genomic instability in the human genome. An amplification of 2.5–4.6 Mb at 20q11.21, encompassing ∼23 genes in common, was detected in four cell lines of different origins. This amplification, which has been associated with oncogenic transformation, may provide a selective advantage to hES cells in culture.
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Gene Expression Omnibus
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This study has been supported in part by additional grants from Medicen Paris Region (IngeCELL), EC (FP6, STEM-HD) and ANR (HESCREEN). The authors thank K. Sermon (AZ-VUB, Brussels) for kindly providing VUB cell lines.
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Lefort, N., Feyeux, M., Bas, C. et al. Human embryonic stem cells reveal recurrent genomic instability at 20q11.21. Nat Biotechnol 26, 1364–1366 (2008). https://doi.org/10.1038/nbt.1509
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