a, RT–PCR detection of MelLec expression in various tissues. The expression of glyceraldehyde-3-phosphate dehydrogenase (G3PDH) in these samples, also used for the characterization of MCL³⁶, is shown as a control. The experiment was performed once; for gel source data, see Supplementary Fig. 1. b, Flow-cytometric analysis of surface expression of haemagglutinin-tagged mouse (m) and human (h) MelLec on the surface of NIH3T3 fibroblasts (black open histograms). NIH3T3 cells transfected with vector only served as controls (grey filled histograms). c, Western-blot analysis of lysates of haemagglutinin-tagged MelLec expressing NIH3T3 cells under reducing and non-reducing conditions and with (+) and without (-) N-glycosidase. Haemagglutinin-tagged CLEC12A (ref. 31) expressing NIH3T3 cells served as controls (for blot source data, see Supplementary Fig. 1). d, Relative binding of FITC-labelled ΔrodA A. fumigatus conidia to NIH3T3 cells transduced with vector only, Dectin-1 or MelLec, as determined by flow cytometry. Values shown are mean ± s.d., analysed by one-way ANOVA. e, Screening of hybridoma supernatants on MelLec-expressing (red) and parental (black) NIH3T3 cells. In b–e, experiments were repeated at least three times independently, with similar results. *P ≤ 0.05; NS, not significant.