Extended Data Figure 4 : MelLec recognizes naphthalene-diol.
From: Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus
a, Detection of MelLec ligands in ghosts of melanin mutants of A. fumigatus by ELISA, as indicated. Values show mean ± s.d. b, Representative immunofluorescence and light micrograph images of Fc-MelLec ligands on NaOH-treated A. fumigatus B5233 conidia after pretreatment with ghosts of ΔpksP or Δayg1 conidia, as indicated. c, Representative immunofluorescence micrographs and light microscopy images of MelLec ligands on NaOH-treated wild-type A. fumigatus conidia after pretreatment with or without YWA1. In a–c, experiments were repeated at least three times independently, with similar results. d, Detection of Fc-MelLec or Fc-Dectin-1 ligands on ΔrodA conidia after pretreatment with (blue) or without (red) YWA1. The experiment was repeated independently twice, with similar results. e, f, Detection of 1,8-DHN (e) and 1,2-DHN and 1,4-DHN (f) by Fc-MelLec and Fc-control using ELISA. Values show mean ± s.d. g, Detection of 1,8-DHN, naphthalene and 1-naphthol by Fc-MelLec using ELISA. Values show mean ± s.d. In e–g, experiments were repeated at least three times independently, with similar results.
