a, b, Cartoon representations of the structures of Fc-MelLec (a) and the full-length receptor (b). Lollipop structures represent predicted glycosylation sites. c, Fc-MelLec or Fc-CLEC12B (Fc-control) staining of C. albicans hyphae, generated in RPMI with 10% fetal bovine serum for 90 min. Fungal particles were analysed by flow cytometry. Experiment was repeated independently twice, with similar results. d, Representative light microscopy images and immunofluorescence micrographs using Fc-MelLec or anti-galactomannan (GM, control) as probes to detect ligands on A. fumigatus mycelium. Experiments were repeated three times independently, with similar results. e, Representative light microscopy images and immunofluorescence micrographs showing the surface distribution of MelLec ligands on C. cladosporioides using Fc-MelLec as a probe. The lower panels show fungal cells after treatment with 1 M NaOH. Experiments were repeated three times independently, with similar results. f, IL-2 production by MelLec-expressing BWZ reporter cells after stimulation by anti-MelLec antibody (αMelLec) crosslinking or with ΔrodA (1:1, 5:1, 10:1) or ΔrodAΔpksP (5:1, 10:1) A. fumigatus conidia, as indicated. Values shown are mean ± s.d. Experiment was repeated three times independently, with similar results.