Figure 2 : MelLec recognizes DHN-melanin.
From: Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus
a, Detection of MelLec ligands in alkali-insoluble and alkali-soluble A. fumigatus cell-wall fractions by ELISA. Values show mean ± s.d. b, Representative histograms showing ΔrodA or ΔpksP A. fumigatus conidia, stained with Fc-MelLec or Fc-CLEC12B (Fc-control) and analysed by flow cytometry. c, The biosynthetic pathway of DHN-melanin (left), and representative immunofluorescence micrographs obtained using Fc-MelLec to detect ligands on A. fumigatus strains deficient in the stated enzymes (right). The conidial rodlet layer was removed with 1 M NaOH before staining. d, Detection of YWA1 by Fc-MelLec and Fc-control by ELISA. Values show mean ± s.d. Experiments were repeated at least three times independently, with similar results.
