a, Survival of corticosteroid-treated mice after i.t. infection with 10⁵ A. fumigatus conidia (n = 15 mice per group). Pooled data from two independent experiments, analysed by log-rank test. b, Fungal burdens in various mouse tissues, as indicated, 4 days after i.v. infection with 10⁶ A. fumigatus conidia (n = 12 mice per group). Values shown are mean ± s.e.m. of pooled data from two independent experiments, analysed by two-sided Mann–Whitney U test. c, Tissue section of kidney from day-4-infected Clec1a^−/− mouse stained with Grocott’s methenamine silver stain and haematoxylin (n = 3 mice per group). d, Immunofluorescence microscopy of brain from day-2-infected Clec1a^−/− mouse (n = 3 mice per group). Fungi are stained with calcofluor white (blue), leukocytes with Gr1 (green) and DNA with propidium iodide (red). e, Fungal burdens in various mouse tissues, as indicated, four days after i.v. infection with 10⁶ ΔpksP A. fumigatus conidia (n = 4 mice per group). Values shown are mean ± s.d., analysed by two-sided Mann–Whitney U test. *P ≤ 0.05; NS, not significant.