Extended Data Figure 8 : Clec1a−/− mice show early inflammatory defects upon challenge with A. fumigatus.
From: Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus
a, Survival (left) and weight measurements (right) of immunocompetent mice after i.t. infection with 107 A. fumigatus conidia (n = 4 mice per group). Values shown are mean ± s.d. b, Exemplar flow-cytometric gating strategy for the identification of CD45+ cells from bronchoalveolar lavage. c, Representative FACS profiles of pulmonary CD11b+ Ly6Ghigh neutrophils in wild-type and Clec1a−/− mice 4 h after challenge with 107 A. fumigatus conidia (wild-type n = 29 mice; Clec1a−/− n = 26 mice). d, e, Pulmonary CD11b+ Ly6Ghigh neutrophils (wild-type n = 29 mice, Clec1a−/− n = 26 mice) (d) and cytokines (n = 25 mice per group) (e) in mice 4 h after challenge with 107 A. fumigatus conidia. f, Pulmonary CD11b+ Ly6Ghigh neutrophils in mice 4 h after challenge with melanin ghosts (160 μg) of A. fumigatus (wild-type n = 15 mice; Clec1a−/− n = 10 mice). Samples with blood contamination were excluded. g, Pulmonary CD11b+ Ly6Ghigh neutrophils in mice 24 h after challenge with 107 A. fumigatus conidia (wild-type n = 33 mice, Clec1a−/− n = 30 mice). h, Cellular inflammatory profiles of mice 24 h after challenge with 107 A. fumigatus conidia (wild-type n = 33 mice, Clec1a−/− n = 30 mice). Alveolar macrophages were defined as CD11c+Siglec-F+, inflammatory macrophages as CD11b+ F4/80+ and eosinophils as CD11b+Siglec-F+. In d–h, values shown are mean ± s.e.m. of pooled data from at least two independent experiments, analysed by two-sided Mann–Whitney U test. i, Expression of MelLec on pulmonary CD45− CD31+ cells isolated from uninfected mice (black) and mice 24 h after infection with A. fumigatus conidia (red). Grey line, isotype control (n = 3 mice per group). j, Pulmonary CD11b+ Ly6Ghigh neutrophils in mice 4 h after challenge with 107 ΔpksP A. fumigatus conidia (wild-type n = 7 mice, Clec1a−/− n = 8 mice). Values shown are mean ± s.e.m. of pooled data from two independent experiments, analysed by two-sided Mann–Whitney U test. k, Representative FACS profiles of pulmonary CD11b+ Ly6Ghigh neutrophils in wild-type and Clec1a−/− mice 4 h after challenge with 107 ΔpksP A. fumigatus conidia (wild-type n = 7 mice, Clec1a−/− n = 8 mice). *P ≤ 0.05; NS, not significant.
