Extended Data Figure 10: Additional behavioural results of eNpHR3.0- or oChIEF-based photostimulation. | Nature

Extended Data Figure 10: Additional behavioural results of eNpHR3.0- or oChIEF-based photostimulation.

From: Ketamine blocks bursting in the lateral habenula to rapidly relieve depression

Extended Data Figure 10

a, Representative trace showing that LHb neurons can follow only the first of the five 100-Hz pulsed blue light stimulations in a pulsed 100-Hz protocol in LHb brain slices infected with AAV2/9-oChIEF. Percentage of responsive neurons shown on the right. bd, Pulsed 100-Hz photostimulation of mice expressing oChIEF does not change locomotion in the OFT (b), and does not induce depressive phenotypes in the FST (c) or SPT (d). n = 6, 7 mice (b, c) and 5, 5 mice (d) for oChIEF and eGFP groups, respectively. e, Representative trace showing LHb neurons following a 5-Hz tonic blue light stimulation protocol in LHb brain slices infected with AAV2/9- oChIEF. Percentage of responsive neurons shown on the right. fh, 5-Hz photostimulation of LHb in mice expressing oChIEF does not change locomotion in the OFT (f) and does not induce depressive phenotypes in the FST (g) or SPT (h). n = 7, 6 mice (f); 6, 6 mice (g) and 5, 12 mice (h) for oChIEF and eGFP groups, respectively. i, 20-Hz tonic photostimulation of the LHb in mice expressing oChIEF does not cause RTPA. Note that this result is different from 20-Hz optogenetic stimulation of a presynaptic input from the entopeduncular nucleus into the LHb, which caused aversion11. n = 5, 7 mice for oChIEF and eGFP group. j, k, eNpHR3.0-driven burst does not change speed of movement in RTPA (j) or affect locomotion in the OFT (k). n = 11, 7 mice (j) and 13, 21 mice (k) for NpHR and eGFP group, respectively. Data are mean ± s.e.m.; n.s., not significant. Two-way ANOVA (b, d, f, h, k), two-tailed unpaired t-test (c, g, i) and paired t-test (j).

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