Extended Data Figure 7: T-VSCC currents and RMPs of LHb neurons in animal models of depression. | Nature

Extended Data Figure 7: T-VSCC currents and RMPs of LHb neurons in animal models of depression.

From: Ketamine blocks bursting in the lateral habenula to rapidly relieve depression

Extended Data Figure 7

a, Voltage steps used to isolate T-VSCC currents, starting from a holding potential of −50 mV before being increased to conditioning potential (−100 mV) for 1 s preceding the command steps (5 mV, 0.1 Hz per step increment). b, LHb T-VSCC currents (right column) are obtained by subtraction of recorded traces without (left) mibefradil from those with mibefradil (10 μM, middle). The maximum of isolated T-VSCC current is obtained at −50 mV. c, d, No difference in LHb T-VSCC currents is detected between wild-type and cLH rats (c) or control and CRS mice (d). n = 4 neurons per group from 2 wild-type and 2 cLH rats (c); n = 5 neurons per group from 2 control and 2 C57 mice (d). e, f, Scatter plots showing that neuronal RMPs are more hyperpolarized in cLH rats (e) and CRS mice (f) than in controls. n = 45 neurons per group from 5 wild-type and 4 cLH rats (e); n = 50 neurons per group from 4 control and 3 C57 mice (f). Data are mean ± s.e.m.; ***P < 0.001, ****P < 0.0001. Two-tailed unpaired t-test (e, f).

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