Extended Data Figure 3 : Isolation and characterization of RGBA-1-derived peptides.

From: Genetic variation in glia–neuron signalling modulates ageing rate

Extended Data Figure 3

a, Separation of N2 worm neuropeptides by HPLC. Total neuropeptides were isolated from the mixture of N2 males and hermaphrodites. Grey bar indicates fractions selected for further analysis. mAU, milli-absorbance unit. bf, Tandem mass spectrometry spectrum of RGBA-1-derived peptides. Peptide sequence was confirmed by higher-energy collisional dissociation fragmentation; y-type and b-type ions are shown in the spectrum.