Extended Data Figure 2 : CRISPR–Cas9-mediated genome editing in rgba-1 and npr-28 genes.

From: Genetic variation in glia–neuron signalling modulates ageing rate

Extended Data Figure 2

a, Sequence confirmation of N2;rgba-13V4H, N2;rgba-13G4R, N2;rgba-13V4R, and CB4856;rgba-13G4H worms. The N2;rgba-13V4H, N2;rgba-13G4R, and N2;rgba-13V4R worms were generated by converting the 3G4H rgba-1 allele to 3V4H, 3G4R, and 3V4R, respectively, in N2 worms. The CB4856;rgba-13G4H worms were generated by changing the 3V4R rgba-1 allele to 3G4H in CB4856 worms. Black arrows indicate SNP sites. b, c, Schematic illustrations of molecular details of rgba-1 (b) and npr-28 (c) mutations. Three nucleotides highlighted in purple represent the protospacer-adjacent motif. d, Sequence confirmation of AB3;npr-28N2, AB3;npr-28166L, N2;npr-28AB3, and N2;npr-28166M worms. The AB3;npr-28166L and AB3;npr-28N2 worms were generated by changing the npr-28 allele to the 166L and N2-type npr-28 allele, respectively, in AB3 worms. N2;npr-28166M and N2;npr-28AB3 worms were generated by changing the npr-28 allele to the 166M and the AB3-type npr-28 allele, respectively, in N2 worms. Black arrows indicate SNP sites.