Extended Data Figure 6 : RNAi screening for BAS-1 regulators identified npr-28.

From: Genetic variation in glia–neuron signalling modulates ageing rate

Extended Data Figure 6

a, Expression of BAS-1::GFP in N2 worms fed with bacteria that express control or npr-28 double-stranded RNAs. Scale bar, 10 μm. Representative of n = 3 independent experiments. b, Predicted transmembrane topology of NPR-28. The variable residue is highlighted in blue. Alignment of NPR-28 with human somatostatin receptor 5 was used for prediction. c, A phylogenetic tree of NPR-28. d, Expression of NPR-28 in serotonergic, dopaminergic, and motor (or inter-) neurons of N2 males. Serotonergic, dopaminergic, and motor (or inter-) neurons were identified by expression of Ptph-1::mCherry, Pdat-1::mCherry, and Pglr-1::mCherry, respectively. Scale bar, 20 μm. Representative of n = 2 independent experiments. e, Mating efficiency of npr-28-null males selectively expressing N2-type NPR-28 in serotonergic, dopaminergic, and motor (or inter-) neurons at day 1 of adulthood. Two independent transgenic lines per genotype were examined. Data shown are mean ± s.e.m. Each data point represents the result of one independent experiment. f, Alignment of a 30-amino-acid sequence centred at the variable 166th residue from NPR-28 of C. elegans and its Caenorhabditis briggsae, Caenorhadbitis remanei, and Caenorhabditis brenneri homologues. The red asterisk indicates the variation site in wild strains of C. elegans.