a, Scatterplot showing SU-pcGBM2 (n = 2) gene expression changes after 16 h of treatment with vehicle (~1% DMSO) or NLGN3 (100 nM). The x axis shows mean fragments per kilobase of transcript per million mapped reads (FPKM) value in vehicle-treated cells, and the y axis shows log2(fold change) of NLGN3 compared to vehicle. Points shown in red represent genes showing statistically significant change (adjusted P < 0.1, Benjaminin–Hochberg for multiple comparison testing). b, Gene Ontology (GO) biological processes enriched in significantly upregulated genes with NLGN3 treatment, as identified by DAVID with P values shown with Benjamini–Hochberg adjustment for multiple comparison testing. c, Genes associated with each GO biological processes terms shown in b.