Maternal immune activation (MIA) contributes to behavioural abnormalities associated with neurodevelopmental disorders in both primate and rodent offspring1,2,3,4. In humans, epidemiological studies suggest that exposure of fetuses to maternal inflammation increases the likelihood of developing autism spectrum disorder5,6,7. In pregnant mice, interleukin-17a (IL-17a) produced by T helper 17 (TH17) cells (CD4+ T helper effector cells involved in multiple inflammatory conditions) induces behavioural and cortical abnormalities in the offspring exposed to MIA8. However, it is unclear whether other maternal factors are required to promote MIA-associated phenotypes. Moreover, the underlying mechanisms by which MIA leads to T cell activation with increased IL-17a in the maternal circulation are not well understood. Here we show that MIA phenotypes in offspring require maternal intestinal bacteria that promote TH17 cell differentiation. Pregnant mice that had been colonized with mouse commensal segmented filamentous bacteria or human commensal bacteria that induce intestinal TH17 cells were more likely to produce offspring with MIA-associated abnormalities. We also show that small intestine dendritic cells from pregnant, but not from non-pregnant, females secrete IL-1β, IL-23 and IL-6 and stimulate T cells to produce IL-17a upon exposure to MIA. Overall, our data suggest that defined gut commensal bacteria with a propensity to induce TH17 cells may increase the risk of neurodevelopmental disorders in the offspring of pregnant mothers undergoing immune system activation owing to infections or autoinflammatory syndromes.
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We thank S. Hang, D. Paik and N. Silverstein for valuable discussions and Y. Yang, M. Xu, N. Geva-Zatorsky, D. Kasper, C. Benoist and D. Mathis for reagents. We thank M. Trombly, N. Silverstein and A. Park for critical reading of the manuscript. We also thank E. Bridge, E. Jaskolski and other staff members at the Department of Animal Medicine at University of Massachusetts Medical School. This work was supported by the Simons Foundation Autism Research Initiative 274443 (D.R.L.) and 402005 (J.R.H.), the Simons Foundation to the Simons Center for the Social Brain at MIT (Y.S.Y., J.R.H and G.B.C.), Hock E. Tan and K. Lisa Yang Center for Autism Research (G.B.C.), the Howard Hughes Medical Institute (D.R.L.), Robert Buxton (G.B.C.), the National Research Foundation of Korea grants MEST-35B-2011-1-E00012 (S.K.) and NRF-2014R1A1A1006089 (H.K.), the Searle Scholars Program (J.R.H.), the Pew Scholar for Biomedical Sciences (J.R.H.), the Kenneth Rainin Foundation (J.R.H.), and the National Institutes of Health grants R01DK106351 and R01DK110559 (J.R.H.).
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Current Neurology and Neuroscience Reports (2018)