Extended Data Figure 7 : Registration of cells across three days of imaging.

From: Social behaviour shapes hypothalamic neural ensemble representations of conspecific sex

Extended Data Figure 7

a, Left, maximum projection maps of spatial filters of all cells from each of three days of imaging for an example mouse. Right, RGB-composite image of the three left images (day 1 = red channel, day 2 = green channel, day 3 = blue channel), showing overlap of registered filters (overlap in all three channels appears in white). Outlined are 20 example cells (out of 135) that could be identified in all three days of imaging: red/green/blue lines indicate filter outlines on first/second/third day, respectively; black points mark filter centroids. b, Histograms of average distance between filter centroids from days 1–3, in cells that could be tracked across days (red) as compared to random triplets of cells (black). Day 1–3 centroids from tracked cells were separated by an average of 2.15 ± 0.06 μm (mean ± s.e.m., n = 593 cells tracked across days in six mice, during the standard resident–intruder assay).