Extended Data Figure 4 : Presynaptic inputs to Lhx6+ zona incerta neurons.

From: Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep

Extended Data Figure 4

Continued from Fig. 1. a, Schematic showing the distribution of presynaptic neurons (red dots) to Lhx6 zona incerta neurons and location of presynaptic neurons across the rostro-caudal gradient of the mouse brain. For mice injected with rabies + TVA (tumour virus A, the receptor for modified rabies virus), n = 4. For rabies only controls, n = 2. bd, Distribution of presynaptic neurons to Lhx6+ zona incerta neurons located in the lateral hypothalamus (RABV, b, red). GABA immunostaining (c, grey) was used to label neurons in the lateral hypothalamus. Merged image is shown in d. eg, Distribution of presynaptic neurons to Lhx6 zona incerta neurons located in the periaqueductal grey (RABV, e, red). GABA immunostaining (f, grey) was used to label neurons in the periaqueductal grey. Merged is shown in g. Arrows indicate neurons with RABV and GABA co-localization (bg). Scale bar, 100 μm. AC, anterior commissural nucleus; AR, arcuate nucleus; Acb, nucleus accumbens; aPAG, anterior periaqueductal grey; APT, anterior pretectal nucleus; ATC, anteromedial thalamic nucleus; BNST, bed nucleus of the stria terminalis; CA1, hippocampus CA1; CA3, hippocampus CA3; CeA, central nucleus of the amygdala; Cg1/Cg2, cingulate cortex; Cl, Claustrum; CM, central medial nucleus of thalamus; CPu, striatum; DG, dentate gyrus; DMH, dorsomedial hypothalamus; DpG, motor-related nuclei of superior colliculus; DR, dorsal raphe nucleus; ECIC, external cortex of the inferior colliculus; Ent, entorhinal cortex; Gi, midline gigantocellular nucleus of the medulla; HDB/VDB, nucleus of the diagonal band; Ing, intermediate grey layer of superior colliculus; Lat, lateral/dentate cerebellar nucleus; LDTG, laterodorsal tegmental nucleus; LH, lateral hypothalamus; LHb, lateral habenula; LO, lateral orbital cortex; LPBE, lateral parabrachial nucleus of the pons; LPOA, lateral preoptic area; LS, lateral septum; LVE, lateral vestibular nucleus; M1/M2, frontal/secondary motor cortex; Med, fastigial nucleus; MePD, posterodorsal medial amygdaloid nucleus; MHb, medial habenula; MM, medial mammillary nucleus; MnPO, median preoptic nucleus; MnR, median raphe nucleus; MPB, medial parabrachial nucleus; MPOA, medial preoptic area; MS, medial septal nucleus; PH, posterior hypothalamus; Pir, piriform cortex; PnO, pontine reticular nucleus, oral part; PPTG, pedunculopontine tegmental nucleus; PVH, paraventricular nucleus of the hypothalamus; PVT, paraventricular nucleus of thalamus; RMG, nucleus raphe magnus; Rn, red nucleus; RS, retrosplenial area; RT, reticular nucleus; RtTg, midbrain reticular formation; S, subiculum; S1/S2, somatosensory cortex; S1BF, barrel cortex; SFO, subfornical organ; SNPC, substantia nigra pars compact; SNR, substantia nigra, reticular part; SPF, parafascicular thalamic nucleus; SuM, supramammillary nucleus; TeA, temporal association cortex; TMN, tuberomammillary nucleus; VLGN, ventrolateral geniculate nucleus; vlPAG, ventrolateral Periaqueductal grey; VMH, ventromedial hypothalamus; VO, ventral orbital cortex; VP, ventral pallidum; VTA, ventral tegmental area; ZI, zona incerta.