Extended Data Figure 2 : Lhx6-Cre activity recapitulates endogenous Lhx6 expression pattern and immunohistochemical analysis of Lhx6-expressing neurons.

From: Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep

Extended Data Figure 2

Continued from Extended Data Fig. 1. ad, Representative images showing Lhx6+ neurons in the zona incerta (a, green), tdTomato expression in the Lhx6-cre;Ai9 line (b, red). tdTomato staining is also observed in endothelial cells, where Lhx6 expression is not detected in adult mouse brains. Merged image is shown in c and magnification is shown in d. Arrows indicate examples of colocalization of Lhx6 and tdTomato expression. e, The percentage Lhx6+tdTomato+ neurons relative to tdTomato+ neurons in the zona incerta in the Lhx6-cre;Ai9 line. n = 3 mice. Data are mean ± s.e.m. fh, Representative images showing MCH+ neurons (f, red) in the lateral hypothalamus and Lhx6–eGFP neurons (g, green) in the zona incerta. Merged image is shown in h. ik, Representative images showing Hcrt+ neurons (i, red) in the lateral hypothalamus and Lhx6–eGFP neurons (j, green) in the zona incerta. Merged image is shown in k. Scale bars, 100 μm (ac, fk) and 25 μm (d).