Extended Data Figure 10 : Additional data from AAV-DIO-hM4Di-injected Lhx6-cre mice.

From: Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep

Extended Data Figure 10

Continued from Fig. 4. a, Schematics showing the construct of AAV-DIO-hM4Di–mCherry virus and the injection sites of virus into the zona incerta of Lhx6-cre mice. b, EEG analysis showing the percentage of time in 2-h bins spent in wake (top), NREM sleep (middle) and REM sleep (bottom) after injection of saline or CNO at ZT17 in hM4Di groups. c, Bout length quantification (top) showing duration of wake, NREM and REM episodes and bout count (bottom) showing sleep–wake transitions with saline or CNO injection in hM4Di groups. d, FFT analysis of EEG power spectrum frequency of NREM stage of CNO-injected AAV-DIO-hM4Di (blue) compared to control (saline-injection, red). e, Grouped EEG frequency data of spectrum band analysis between saline- (red) and CNO-injected (blue) hM4Di groups. f, FFT analysis of EEG power spectrum frequency of NREM stage of CNO-injected AAV-DIO-hM4Di (blue) compared to control (saline-injection, red). g, Grouped EEG frequency data of spectrum band analysis between saline- (red) and CNO-injected (blue) hM4Di groups. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001; two-way ANOVA followed by Sidak’s post hoc test. n = 8 mice.