Extended Data Figure 8: Polylox barcoding of haematopoiesis in adult mice (all data from adult mice with barcodes induced as adults). | Nature

Extended Data Figure 8: Polylox barcoding of haematopoiesis in adult mice (all data from adult mice with barcodes induced as adults).

From: Polylox barcoding reveals haematopoietic stem cell fates realized in vivo

Extended Data Figure 8

a, Barcodes were induced by tamoxifen treatment of adult Rosa26PolyloxTie2MCM mice, and the indicated cell populations were analysed at 11–13 months of age. b, Tamoxifen treatment of adult Rosa26PolyloxTie2MCM mice (Extended Data Table 1) induced Polylox recombination in HSCs and, to a lesser extent, also in downstream stem and progenitor cells, ST-HSCs, MPPs and CMPs (Supplementary Fig. 1e) c, Heatmaps of barcodes satisfying single-cell induction criteria (at the time of labelling) recovered in the indicated stem and progenitor cells, and mature cells in Exp. 3 (left) and Exp. 4 (right). Frequencies of barcodes are represented by colour code (left). d, Heatmaps for individual HSCs satisfying adult single-cell barcode induction criteria, and their lineage output in Exp. 3 (top) and Exp. 4 (bottom). Pgen for the multilineage barcodes were as follows: 1F92G45H3, 1.3 × 10−9; 123FG45, 2 × 10−5. e, The barcode overlap between two samples of the same cell population (granulocytes and CD4 T cells isolated from the peripheral blood; 30,000 cells per sample) was smaller than for embryonically labelled mice (Fig. 4c). f, Hierarchical clustering of rank correlations of barcodes from the indicated populations (Exp. 3, n = 129). The colour scale (not shown) for rank correlations is identical to the scale bar shown in Fig. 5i.

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