a–d, Confocal images of three-day-old roots from seedlings of pSCR::SCR:YFP, pSHR:SHR:YFP, pJKD::JKD:YFP and pNUC::NUC:YFP. Scale bar, 20 μm. e, Box plots showing differences in YFP intensity (16-bit) of roots expressing endogenous fusions of SCR (n = 83), SHR (n = 86), JKD (n = 62) and NUC (n = 24). n represents the number of measured nuclei. The box plot displays the distribution of fluorescence intensity: median, 1st and 3rd quartiles, minimum and maximum. The error bars indicate minimum and maximum of distribution.