Extended Data Figure 8 : Effects of additional ligands on saturation concentrations.

From: Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin

Extended Data Figure 8

a, Bar graphs displaying the effects of 100 μM of the polyamine spermine along with the H3K9 and H3K9me3 peptides on phase-separation behaviour. b, Schematic of the assay used to quantify the partitioning of Cy3-labelled substrates into the two phases. The blue bars represent the total concentration of the labelled species before spin down; the orange bars represent the concentration of Cy3-labelled species remaining in the upper phase after spin down. The lower phase contains HP1α at a higher concentration than in the upper phase. Error bars represent standard error of the mean from three independent measurements. c, Model for phosphorylation or DNA-driven HP1α phase separation. Phosphorylation or DNA binding relieves intra-HP1 contacts and opens up the dimer. The location(s) of the intra- and inter-dimer contacts that change during this transition are not fully understood, but are predicted to involve interactions between the CTE, hinge and NTE.