Extended Data Figure 6: ACSS2 functions in neuronal histone acetylation. | Nature

Extended Data Figure 6: ACSS2 functions in neuronal histone acetylation.

From: Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory

Extended Data Figure 6

a, Western blot analysis of whole-cell lysates shows that lentiviral shRNA-mediated knockdown of ACSS2 lowers H3K9 and H3K27 acetylation (compare to Fig. 2g), quantified using ImageJ (n = 3, error bars show s.e.m.). b, Western blot analysis of eluates and supernatants of IgG control and ACSS2 co-immunoprecipitation experiments indicates that ACSS2 binds to acetylated chromatin. c, Western blots of lysates from primary hippocampal neurons (d7) treated for 24 h with the ACSS2i, probed with the indicated antibodies (compare to Fig. 2j), and quantified using ImageJ (n = 3, error bars show s.e.m.).

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