Extended Data Figure 5 : Effect of laser stimulation on transition probability between each pair of brain states in GABAPOA→TMN-ChR2, GABAPOA→TMN-Ctrl, and GABAPOA-ChR2 mice.

From: Identification of preoptic sleep neurons using retrograde labelling and gene profiling

Extended Data Figure 5

a, Schematic showing transition probability calculation. To calculate the transition probability at a given time bin (i), we first identified all the trials (n) in which the animal was in state X (X could be wake, NREM, or REM) in the preceding time bin (i − 1). Among these n trials, we identified the subset of trials (m) in which the animal transitioned into state Y in the current time bin (i). The XY transition probability for time bin i was computed as m/n. b, Transition probability within each 10 s period in GABAPOA→TMN-ChR2 mice (n = 9). Shown in each bar is the transition probability averaged across six consecutive 10 s bins within each 60 s. Error bar, 95% confidence interval (bootstrap). The baseline transition probability (grey dashed line) was averaged across all time bins after excluding the laser stimulation period. Direct wake→REM and REM→NREM transitions were not observed and the corresponding plots were omitted. Magenta asterisk (*)/green hash symbol (#) indicates significant increase/decrease in transition probability during laser stimulation compared with the baseline (P < 0.05, bootstrap). Top right diagram indicates transition probabilities that are significantly increased (magenta), decreased (green), or unaffected (black) by laser stimulation. c, Transition probability in control mice (n = 8). The probability during laser stimulation was not significantly different from baseline for any transition. d, Transition probability in GABAPOA-ChR2 mice (n = 5).