Extended Data Figure 9: MSI2 and BCAT1 expression in human cancer. | Nature

Extended Data Figure 9: MSI2 and BCAT1 expression in human cancer.

From: Cancer progression by reprogrammed BCAA metabolism in myeloid leukaemia

Extended Data Figure 9

a, Microarray data analysis of MSI2 expression in human CML. Gene expression data of chronic (grey, n = 57), accelerated (pink, n = 15) and blast crisis (blue, n = 41) phase patients were retrieved from the GEO database (accession number GSE4170). Bar, the normalized expression value in each specimen. b, Co-expression analysis of the BCAT1 and MSI2 genes in human cancer. Pearson correlation coefficients were used to evaluate the extent of co-expression patterns. c, Schematic of the human BCAT1 transcript. Bars, the putative MBEs (r(G/A)U1–3AGU). Forty MBEs were identified within the 3′ UTR of BCAT1. CDS, coding sequence for hBCAT1 protein. d, K562 cells infected with lentiviral shRNA against MSI2 (shMSI2) or shCtrl (−) were analysed by western blotting for pS6 kinase (at Thr389), total S6K, hMSI2 and HSP90 levels. Note that MSI2 knockdown reduced the levels of BCAT1 protein and pS6K.

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