Extended Data Figure 4 : Lineage tracing of the Cdh5(PAC)-CreERT2 transgene in neonatal mice.

From: Endothelial TLR4 and the microbiome drive cerebral cavernous malformations

Extended Data Figure 4

ac, R26-LSL-RFP, R26-CreERT2-R26-LSL-RFP, and Cdh5(PAC)-CreERT2-R26-LSL-RFP neonates were induced with doses of tamoxifen on P1+2 (two total doses) and CD45+RFP+ haematopoietic cell numbers in the spleen and peripheral blood were assessed at P10. n ≥ 5 per group. Error bars shown as s.e.m. and significance determined by one-way ANOVA with Holm–Sidak correction for multiple comparisons. ***P < 0.001; n.s., P > 0.05. Note, the number of labelled haematopoietic cells in Cdh5(PAC)-CreERT2-R26-LSL-RFP animals is indistinguishable from R26-LSL-RFP negative control animals, whereas >90% of CD45+ cells were RFP+ in R26-CreERT2-R26-LSL-RFP positive control animals. d, Anti-RFP and anti-PECAM immunostaining of P10 hindbrains from Krit1fl/fl-R26-LSL-RFP-negative control and Krit1ECKO-R26-LSL-RFP was performed to identify Cre+ descendants at the site of CCM formation. Note that all RFP+ cells in Krit1ECKO-R26-LSL-RFP animals are PECAM+, consistent with endothelial-specific Cre activity. Asterisk indicates CCM lesion. Results are representative of ≥3 per group. Scale bars, 100 μm.