Oxetanocin A (OXT-A) is a potent antitumour, antiviral and antibacterial compound. Biosynthesis of OXT-A has been linked to a plasmid-borne Bacillus megaterium gene cluster that contains four genes: oxsA, oxsB, oxrA and oxrB. Here we show that both the oxsA and oxsB genes are required for the production of OXT-A. Biochemical analysis of the encoded proteins, a cobalamin (Cbl)-dependent S-adenosylmethionine (AdoMet) radical enzyme, OxsB, and an HD-domain phosphohydrolase, OxsA, reveals that OXT-A is derived from a 2′-deoxyadenosine phosphate in an OxsB-catalysed ring contraction reaction initiated by hydrogen atom abstraction from C2′. Hence, OxsB represents the first biochemically characterized non-methylating Cbl-dependent AdoMet radical enzyme. X-ray analysis of OxsB reveals the fold of a Cbl-dependent AdoMet radical enzyme, a family of enzymes with an estimated 7,000 members. Overall, this work provides a framework for understanding the interplay of AdoMet and Cbl cofactors and expands the catalytic repertoire of Cbl-dependent AdoMet radical enzymes.
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This work was supported by National Institute of Health Grants F32-GM108189 (J.B.-R.) and GM035906 (H.-w.L.), and the Welch Foundation grant F-1511 (H.-w.L.). C.L.D. is a Howard Hughes Medical Institute Investigator. This work is based upon research conducted at the Northeastern Collaborative Access Team beamlines, which are funded by the National Institute of General Medical Sciences from the National Institutes of Health (P41 GM103403). The Pilatus 6M detector on 24-ID-C beam line is funded by a NIH-ORIP HEI grant (S10 RR029205). This research used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357.
The authors declare no competing financial interests.
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Extended data figures and tables
Extended Data Figure 2 In vivo gene expression and HPLC analysis reveal oxsA and oxsB are required for OXT-A biosynthesis.
a, To probe which genes located within the BglII-D fragment are responsible for OXT-A (chemical structure shown in left panel) production, B. megaterium NRS 269 was transformed using the E. coli–Bacillus shuttle vector pMM1522. b, In vivo product profiles of B. megaterium NRS 269 strains transformed with pMM1522 empty vector (as a control) (i) or transformed with pMM1522 that contains the BglII-D (oxsA, oxsB, oxrA, oxrB) fragment (ii). c, In vivo product profiles of B. megaterium NRS 269 strains carrying only oxsB (i), carrying both oxsA and oxsB (ii), or carrying only oxsA (iii).
High-performance liquid chromatography (HPLC) analysis in panel a was performed using a CarboPac PA1 Dionex column whereas a C18 column was used in panels c–e. a, HPLC analysis of the reactions catalysed by OxsA and OxsB using dAMP, dADP, or dATP as substrate. Incubation with reconstituted OxsB and OxsA with dAMP, DTT, AdoMet, HO-Cbl, MgCl2, NADPH, MV (full reaction, see Methods for details) (i); full reaction without OxsB (ii); full reaction without OxsA (iii); full reaction without dAMP (iv); full reaction without AdoMet (v); full reaction without HO-Cbl (vi); full reaction without the reductants NADPH and MV (vii); full reaction substituted with dADP instead of dAMP (viii); full reaction substituted with dATP instead of dAMP (ix). b, Mass spectrometry (MS) (ESI positive) used to confirm the identity of compound 2 as the corresponding aldehyde of OXT-A 5′-monophosphate. MS of the aldehyde compound was performed following treatment of the reaction mixture with CIP and purification by HPLC. c, HPLC analysis confirms that reduction of compound 2 results in formation of OXT-A-P (3). Isolated 2 (i); isolated 2 treated with NaBD4 (ii); co-injection of 2 treated with NaBD4 with a chemically synthesized standard of 3 (iii); standard of 3 (iv). d, Direct formation of OXT-A is observed when cell extract is included in the reaction conditions. HPLC analysis after incubation of reconstituted OxsB with OxsA, dATP, DTT, AdoMet, HO-Cbl, MgCl2, NADPH, MV, and cell extract of B. megaterium NRS 269 (full reaction) (i); full reaction without OxsB (ii); full reaction without OxsA (iii); full reaction without dATP (iv); full reaction without AdoMet (v); full reaction without MgCl2 (vi); full reaction without cell extract (vii); OXT-A (1) standard (viii). e, Direct formation of OXT-A can also be observed when alcohol dehydrogenase is included in the reaction mixture. HPLC analysis after incubation with reconstituted OxsB and OxsA with dATP, DTT, AdoMet, HO-Cbl, MgCl2, NADPH, MV and horse liver alcohol dehydrogenase (full reaction) (i); full reaction without OxsA and OxsB (ii); OXT-A standard (iii).
a, Consistent with its classification as an AdoMet radical enzyme, OxsB catalyses the reductive cleavage of AdoMet to generate 5′-dAdoH. HPLC analysis (C18 column, 2–20% CH3CN in 1% NH4OAc linear gradient elution) of reaction catalysed by OxsA and OxsB. Reaction of reconstituted OxsB with OxsA, dAMP, DTT, AdoMet, HO-Cbl, MgCl2, NADPH, MV (full reaction, see Methods for details) (i); full reaction without dAMP (ii); 5′-dAdoH standard (iii). b, MS spectrum (ESI positive) of 5′-dAdoH generated in the OxsA and OxsB reaction using [2′-2H2]-2′-dAMP as substrate shows incorporation of the deuterium label into 5′-dAdoH and thus indicates hydrogen atom abstraction occurs at C2′. The less than full deuterium incorporation is probably due to the co-occurrence of uncoupled quenching of 5′-dAdo•, a common phenomenon in many AdoMet radical enzymes40. c, MS spectrum (ESI positive) of OXT-A (1) generated in the OxsA and OxsB reaction using [3′-2H]-2′-dAMP as substrate, which shows retention of the deuterium label in product, again consistent with hydrogen atom abstraction at C2′.
a, A stereoview of the entire (744 amino acids) monomer of OxsB coloured by domain. The N-terminal domain is shown in yellow, and is followed by the Cbl-binding domain displayed in pink, the AdoMet radical domain, which is coloured cyan, and the C-terminal helix bundle domain displayed in blue. b, A topology diagram of OxsB is shown and coloured as in panel a. The yellow sphere in domain II represents the position of Asn186, which is the closest residue to the Co of Cbl. c, The two observed conformations of AdoMet (cyan and wheat), Cbl, and the [4Fe–4S] AdoMet radical cluster (orange and yellow spheres) are shown with simulated annealing composite omit electron density maps contoured at 0.8σ.
a, A stereoview of an overlay of the MetH23 Cbl-binding domain (green) with the Cbl-binding domain of OxsB (pink) shows differences in the positioning of the Cbl cofactor’s corrin ring and the length of the II-β1 loop, which for OxsB lacks a His residue to ligate Cbl. b, Residues from the Cbl-binding domain of OxsB that accommodate or make contact with the DMB tail and corrin ring of Cbl are highlighted and shown as sticks. Residues Gly216 and Ser184 are from the base-off consensus sequence and residues R135–S139 are located on the II-β1 loop. c, Residues from the Cbl-binding domain of MetH23 that interact with Cbl or make room for the DMB tail are highlighted and shown as sticks. d, Residues from panels b and c that make contact with Cbl from the Cbl-binding domains of MetH (top panel) and OxsB (bottom panel) are shown. Residues highlighted in pink are previously identified sequence fingerprints of MetH that have a conserved interaction in OxsB. Residues highlighted in blue are conserved interactions between Cbl and MetH or OxsB, which are not from the standard Cbl-binding motifs. Residues shown in black form interactions with Cbl, but are not conserved between the proteins. e, Stereoview of the contacts that Cbl makes with the Cbl-binding (pink) and AdoMet radical (cyan) domains of OxsB. All of the residues that form interactions with Cbl are highlighted as sticks, but only residues from the AdoMet radical domain are labelled for clarity.
a–d, Small conformational changes occur in the reconstituted structures relative to the apo-structure. The r.m.s.d. determined by PyMOL for the OxsB–Cbl/[4Fe–4S]/AdoMet structure compared to the apo structure is 0.98 Å for 4,770 atoms. a, The II-β1 loop of the Cbl-binding domain in the reconstituted OxsB structures swings outward 12.9 Å to avoid steric clashes with the corrin ring of Cbl and now caps the side of the Cbl. b, c, In the absence of a [4Fe-4S] cluster, the Cys residues of the cluster-binding loop in the apo-structure are oriented similarly to those in the reconstituted structure. Cys318 and Cys321 in the apo-structure, however, exhibit a partial occupancy disulfide linkage. At the end of the cluster binding loop, there are more substantial differences between the structures; His325 and Lys326 of OxsB–Cbl/[4Fe–4S]/AdoMet move 8.0 and 8.8 Å, respectively, from their positions in the apo structure to interact with the nucleotide tail of Cbl. d, An overlay of OxsB–Cbl/[4Fe–4S]/AdoMet (light colours) with apo-OxsB (dark colours) shows slight movements in each domain. The arrow indicates closing in of the helix bundle domain towards the cofactors. e, A surface representation of OxsB reveals the open and solvent accessible nature of the active site in OxsB–Cbl/[4Fe–4S]/AdoMet. Water molecules are shown as red spheres. f, Location of polar and aromatic residues near the active site. Presumably, positively charged residues are needed to accommodate the negatively charged phosphate moieties of substrate and an aromatic residue may stack with substrate adenine. g, An overlay of the β-strands from the AdoMet radical domains of OxsB (cyan) with those from a peptide-bound (green) stucture of PFL-AE25 (grey) was used to map the approximate substrate-binding site in OxsB. The yellow sphere, which corresponds to the Cα of the substrate peptide Gly residue, is 3.7 Å away from the 5′ carbon of AdoMet and 3.7 Å away from Co of Cbl.
a, A stereoview of the AdoMet radical domain of OxsB. Each of the AdoMet radical motifs26,27 is highlighted including the GGE (E363), ribose (E436), GXIXGXXE (I474), and the β6 (adenine-binding) motif (E545). The GGE motif provides a carbonyl to hydrogen bond with the amino moiety of AdoMet. In OxsB, E363 also contacts a Cbl acetamide. The ribose motif is found at the C-terminal loop following III-β4 where Glu436 forms two hydrogen bonds with the AdoMet ribose hydroxyl moieties. In the same loop, two residues upstream, the backbone amide of Gly434 contributes a hydrogen bond to the carboxyl group of AdoMet. Following a short III-α4a helix that connects III-β4 and III-α4, Lys448 interacts with the AdoMet carboxylate similar to what was previously observed in QueE41, HemN34, HydE42, PylB43, anSMEcpe44, and BtrN28. In terms of the GXIXGXXE motif, Ile474 from a loop following III-β5 provides hydrophobic contacts to the adenine ring of AdoMet as observed previously. However, instead of the backbone of Ile474 being stabilized by a polar residue on III-α5, as found in other AdoMet radical enzymes, the backbone of Ile474 is stabilized through interactions with the side chains of Gln442 and Tyr446 from III-α4a and the backbone of ribose motif residue Glu436. The final motif, the so-called β6 motif, is present although β6 is not. A loop substitutes for β6, with backbone atoms of E545 making hydrogen bonds to the adenine ring of AdoMet. Additional residues F320, M544, I546 and L547 that provide hydrophobic interactions to the adenine ring of AdoMet and a hydrogen bond to N6 are also shown. b, A 2Fo − Fc simulated annealing composite omit electron density map contoured at 1.0σ around the AdoMet radical [4Fe–4S] cluster. c, The radical-competent orientation of AdoMet ligates the unique Fe of the AdoMet radical [4Fe–4S] cluster, which is shown rotated approximately 90° from b. The distance between the unique Fe and the AdoMet amino and carboxylate moieties measure 2.2 Å each. d, The non-radical competent orientation also ligates the AdoMet radical [4Fe–4S] cluster with the amino and carboxylate moieties. These distances measure 2.3 and 2.0 Å, respectively. e, A 2Fo − Fc simulated annealing composite omit electron density map contoured at 1.0σ around the two orientations of AdoMet. f, A simulated annealing composite omit electron density map calculated after the radical-competent orientation of AdoMet was omitted from the refined structure of OxsB–Cbl/[4Fe–4S]/AdoMet. This map is contoured at ± 3.0σ around the radical-competent orientation of AdoMet. g, A similar simulated annealing composite omit electron density map was calculated after the non-radical-competent orientation of AdoMet was omitted from the refined structure of OxsB–Cbl/[4Fe–4S]/AdoMet. This map is also contoured at ± 3.0σ around the observed AdoMet conformation.
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Bridwell-Rabb, J., Zhong, A., Sun, H. et al. A B12-dependent radical SAM enzyme involved in oxetanocin A biosynthesis. Nature 544, 322–326 (2017). https://doi.org/10.1038/nature21689
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