Extended Data Figure 6 : Metabolic effects of CPT1AKD in LECs.

From: The role of fatty acid β-oxidation in lymphangiogenesis

Extended Data Figure 6

ae, In vitro experiments with pLECs at baseline (ctrl) and with silencing of CPT1A (CPT1AKD) versus VECs. a, Energy charge (n = 5). b, Percentage of oxidized glutathione (GSSG) per total glutathione (GSSG+GSH) (n = 3). c, Relative NADP+/NADPH ratio, expressed relative to ctrl VECs (n = 3). d, CM-H2DCFDA (intracellular ROS) signal (n = 3). e, H2O2 levels, as detected with Amplex Red reagent (n = 3). fk, In vitro experiments with CPT1AKD pLECs versus ctrl pLECs. f, Percentage total contribution of [U-13C]palmitate carbons to citrate (Cit), α-ketoglutarate (αKG), fumarate (Fum) and malate (Mal) (n = 3). g, Cellular pool size of citrate), α-ketoglutarate, fumarate and malate, measured as arbitrary units (AU) of the metabolite of interest normalized to the protein content (n = 3). h, Relative incorporation of [U-14C]-palmitate carbons into DNA (n = 3). i, Percentage total contribution of [U-13C]palmitate carbons to aspartate (Asp), glutamate (Glu), UTP and CTP (n = 3). j, Cellular pool size of aspartate, glutamate, UTP, CTP, ATP and GTP, measured as arbitrary units (AU) of the metabolite of interest normalized to the protein content (n = 3). k, Total sprout length from spheroid sprouting assay in pLECs upon CPT1AKD, without (ctrl) and with deoxyribonucleotide (dNTP) supplementation versus pLECs without CPT1AKD (ctrl). Note, the first two bars are the same data as Fig. 6e, performed in the same independent experiment (n = 3). Mean ± s.e.m. of n independent experiments. Statistical test: ANOVA and Bonferroni post-hoc test were used in multiple group comparisons. t-test was used for comparison of two groups. *P < 0.05; NS, not statistically significant.