a, The ilc-17.1 mutants expressing ilc-17.1 cDNA from the hsp-16.41 promoter assayed without heat shock. Assays were performed 2 h after animals were picked to assay plates; n = 4 assays, 120 animals. *** P < 0.001, Mann–Whitney U-test. b, c, Timelines showing mCherry expression ( b) and speed at 21% O 2 ( c) of ilc-17.1 mutants bearing a phsp-16.41::ilc-17.1::mCherry bicistronic transgene and heat shocked for 15 or 30 min. For each mCherry measurement, n = 24; speed in c is the average of a 1-min time window, 40 s after switching to 21% O 2 and 20 s before switching back to 7% O 2, indicated with a red bar in d and e. The dashed lines in c indicate the average speed of npr-1 (black) and ilc-17.1 npr-1 (blue) animals for comparison. ** P < 0.01, *** P < 0.001, Mann–Whitney U-test; P values in black are for comparisons with npr-1 control; P values in blue are for comparisons with ilc-17.1 npr-1 controls. d, e, Transgenic animals expressing ilc-17.1 cDNA from the hsp-16.41 promoter were exposed to 34 °C for 15 min ( d) or 30 min ( e), then assayed every 2 h. Statistical comparisons between npr-1 and transgenic animals with heat-shock constructs are indicated in orange. Comparisons between npr-1 ilc-17.1 and transgenic animals with heat-shock constructs are indicated in black; n = 4 assays, 120 animals. *** P < 0.001, ** P < 0.01, * P < 0.05, Mann–Whitney U-test.