Extended Data Figure 2 : ILC-17.1 signalling in RMG promotes aggregation and escape from 21% O2.

From: IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses

Extended Data Figure 2

a, b, The bordering and aggregation defects of ilcr-1 and ilcr-2 mutants can be rescued by selectively expressing their cDNAs in RMG interneurons (pnpr-1 and pflp-5). Bordering but not aggregation is partly rescued by expression in the O2-sensing neurons URX, AQR, and PQR (pgcy-32); n = 4 assays. *P < 0.05, **P < 0.01, ****P < 0.0001, ANOVA with Tukey correction. c, d, O2 responses of ilcr-1 (c) and ilcr-2 (d) mutants expressing the corresponding cDNA selectively in RMG (pnpr-1 and pflp-5), the O2 sensing neurons URX, AQR, and PQR (pgcy-32), or the ASE gustatory neurons (pflp-6); n = 4 assays, 120 animals. ***P < 0.001, Mann–Whitney U-test. e, Expressing ilcr-1 or pik-1 cDNA in ASG neurons, using the ops-1 promoter, fails to rescue the O2 response defects of the corresponding mutants; n = 4 assays, 120 animals. ***P < 0.001, Mann–Whitney U-test. f, Expressing ilcr-1 cDNA in cholinergic neurons (punc-17 or pacr-2), GABAergic neurons (punc-25), or interneurons controlling forward and reverse movement (pglr-1, pnmr-1) fails to restore sustained locomotory arousal to ilcr-1 mutants kept at 21% O2; n = 4 assays, 120 animals. ***P < 0.001, Mann–Whitney U-test. g, Expressing ilc-17.1 cDNA in RMG using the flp-5 promoter rescues defective responses to 21% O2 in ilc-17.1 mutants. Speed assays were performed 2 h after animals were transferred to the assay plates; n = 4 assays, 120 animals. ***P < 0.001, Mann–Whitney U-test. h, Expressing ilc-17.1 cDNA from the odr-10 promoter (AWA) or the flp-6 promoter (ASE) not only rescues the ilc-17.1 defect but confers abnormally heightened escape from 21% O2; n = 4 assays, 120 animals. **P < 0.01, ***P < 0.001, Mann–Whitney U-test. i, Expressing ilcr-2 cDNA using the flp-5 promoter rescues the RMG O2-evoked Ca2+ responses defects of ilcr-2 mutants. ***P < 0.001, Mann–Whitney U-test. The npr-1 control is the same as in Fig. 4e. j, The ilc-17.1; npr-1 215F and nfki-1; npr-1 215F mutants show defective O2-evoked Ca2+ response in RMG; npr-1 215F is the allele found in natural isolates of C. elegans. ***P < 0.001, Mann–Whitney U-test. k, Expression of pflp-5::GFP in RMG interneurons, a reporter of RMG neurosecretory activity, is reduced in ilc-17.1, ilcr-1, and ilcr-2 mutants. ***P < 0.001, ANOVA with Tukey correction. l, m, The ilc-17.1, pik-1, and nfki-1 mutants display normal chemotaxis to NaCl (l) and benzaldehyde (m); n = 6 assays. NS, not significant, ANOVA with Tukey correction. n, Ca2+ responses evoked in AIB interneurons by benzaldehyde (1:10,000 dilution) are normal in ilc-17.1 mutants; bz, benzaldehyde; Mann–Whitney U-test.