Extended Data Figure 9 : Mutations in the nfki-1 gene cause ilc-17.1-like phenotypes.

From: IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses

Extended Data Figure 9

a, b, The nfki-1 alleles. Allele db923 in strain AX3677 was obtained in an EMS screen (a); the db1198 frameshift mutation was generated using CRISPR (b). c, A functional pnfki-1::nfki-1::SL2::mCherry polycistronic transgene is expressed broadly in the nervous system. RMG expression was confirmed using a pflp-5::gfp fiduciary marker. d, e, Phenotypes of nfki-1, pik-1, and ilc-17.1 molecular null alleles are not additive; n = 4 assays, 120 animals. ***P < 0.001, Mann–Whitney U-test. f, Schematic of the human IκBζ b isoform and NFKI-1, highlighting ankyrin repeats (ANK). g, Alignment of amino-acid sequences for IκBζ orthologues from different species. NFKI-1 is shown at the bottom. Conserved residues are highlighted.