Extended Data Figure 7 : The C. elegans Act1-like gene.

From: IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses

Extended Data Figure 7

a, b, The actl-1 alleles. Allele db789 was isolated in strain AX3544 after mutagenesis using ethylmethanesulfonate (EMS) (a); db1202 and db1203 are frameshift mutations generated by CRISPR (b). c, Mos1-mediated single-copy insertion (MosSCI) of the actl-1, pik-1, and nfki-1 genes rescues the O2 response defects of the corresponding mutants; n = 4 assays, 120 animals. ***P < 0.001, **P < 0.01, Mann–Whitney U-test. d, A null mutation in actl-1 confers ilc-17.1-like responses to 21% O2, and does not show additive phenotypes with ilc-17.1 or pik-1 null mutations; n = 4 assays, 120 animals. ***P < 0.001, Mann–Whitney U-test. e, Domain architecture of ACTL-1, showing the locations of the Death and SEFIR domains. f, Alignment of the SEFIR domains of ACT1 and ACT1-like proteins. C. elegans ACTL-1 is shown at the top.