Extended Data Figure 2 : Cryo-EM image processing procedure.

From: Structure of a CLC chloride ion channel by cryo-electron microscopy

Extended Data Figure 2

a, A representative micrograph of the CLC-K–Fab complex (scale bar, 50 nm). Boxed regions (white squares) are magnified on the right to show representative particle images. Note that in the top/bottom views, Fab signals are evident while the channel part is barely visible because of low contrast (also see b). b, Images of selected two-dimensional classes from reference-free two-dimensional classification by RELION. Note that the distal half of the Fab fragment shows much flexibility, resulting in blurred features. Scale bar, 10 nm. c, Summary of the image processing procedure (see Methods).