Extended Data Figure 8 : Density in the Scen Cl-binding site.

From: Structure of a CLC chloride ion channel by cryo-electron microscopy

Extended Data Figure 8

A view into Scen of CLC-K (from Sext towards the cytosol) is shown in a stereo diagram. Predicted polar interactions between bound Cl ions and the protein, including anion–quadrupole interactions, are indicated by green dashed lines. The shown EM density map is of the class 1 structure, sharpened with a B-factor of −100 Å2 and low-pass filtered at 3.7 Å. Density for protein (grey mesh) and possible ions (magenta mesh) are shown at contour levels of 5.8σ and 3.2σ, respectively.