Extended Data Figure 2 : Enhanced recurrent weight gain after initial obesity.

From: Persistent microbiome alterations modulate the rate of post-dieting weight regain

Extended Data Figure 2

a, b, The effect of celastrol on weight loss in mice continuously fed a HFD (a) and mice with alternating diets (b). ce, Weight regain quantification by AUC (c), weight regain slope (d,), and net weight gain on HFD (e) by control mice and weight-cycling mice treated with celastrol to lose weight. f, g, Quantification of weight regain by AUC (f), weight regain slope (g) of leptin antagonist-treated weight-cycling mice and controls. h, i, Body fat content (h), and serum cholesterol levels (i) of weight-cycling mice undergoing a third weight cycle and controls. j, Schematic of the analysed time point in ko. k, Weight gain during 4 weeks of HFD. lo, Glucose levels after glucose tolerance test (l), glucose level quantification (m), serum cholesterol levels (n), and quantification of body fat content (o) in weight-cycling mice during initial obesity and controls. Experiments were repeated twice. Shown are mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by ANOVA (c, f, h, i, m, n, o) or Mann–Whitney U-test (d, e, g). See Supplementary Tables 5 and 6 for exact n and P values.