Extended Data Figure 1 : Metabolic measurements during weight regain.

From: Persistent microbiome alterations modulate the rate of post-dieting weight regain

Extended Data Figure 1

a, Schematic indicating time point of metabolic measurements. b, Quantification of weight regain by weight gain. c, Net weight gain induced by 8 weeks of HFD in weight-cycling mice and continuous HFD. d, e, Coronal (above) and axial (below) MRI scans (d), and quantification of body fat content (e). fh, Serum levels of leptin (f), LDL (g), and HDL (h) during the second HFD exposure of mice undergoing weight cycling and controls. i, j, Quantification of dark phase (i) and light phase (j) energy expenditure upon weight regain of weight-cycling mice. kq, Representative recordings (k, n, q) and quantifications (l, m, o, p) of O2 consumption (km), CO2 consumption (np), and food intake (q) upon weight regain of weight-cycling mice. Experiments were repeated twice. Shown are mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by ANOVA (ep) or Mann–Whitney U-test (b, c). See Supplementary Tables 5 and 6 for exact n and P values.