Extended Data Figure 4 : Fluorescence anisotropy data for bat FluA polymerase.

From: Structural basis of an essential interaction between influenza polymerase and Pol II CTD

Extended Data Figure 4

a, Displacement of a fluorescent SeP5 CTD peptide (four-repeat) bound to Pol–vRNA complex with non-labelled SeP5 peptide. The apparent Kd (Kd′) is shown. b, Binding of SeP5 peptide to FluA polymerase in the absence of the vRNA promotor. c, Interaction with non-phosphorylated four-repeat CTD peptide (Y1S2P3T4S5P6S7). The binding curve can be extrapolated to an estimated Kd of >10 μM. Error bars represent s.d. of three independent experiments, Kd values are shown ± the error of fit.