Extended Data Figure 9 : Overlap between a functionally important region of FluA PA defined by fitness profiling and the CTD-binding site.

From: Structural basis of an essential interaction between influenza polymerase and Pol II CTD

Extended Data Figure 9

a, b, A putative functional subdomain (a) identified in figure 5 of ref. 27 partially overlaps with the two CTD-binding sites (b). Amongst the residues shown to be functionally important in ref. 27, K559, R454 and K635 (K554, R449 and K630 bat FluA numbering) are directly involved in the interaction with the CTD peptide (see Extended Data Fig. 3). The authors also showed that R454 and K635 mutations essentially eliminated polymerase activity in a minigenome assay, consistent with our results.