Extended Data Figure 6 : Minigenome assays for CTD-binding mutants of influenza A/WSN/33 in absence of NS2.

From: Structural basis of an essential interaction between influenza polymerase and Pol II CTD

Extended Data Figure 6

a, Overall RNP activity measured by a luciferase reporter assay (upper panel). Expression of PA, PB2 and nucleoprotein subunits is monitored by western blot (lower panel). b, Primer extension assay showing the different RNA levels produced by wild-type and mutant polymerases in the minigenome assay. Products of primer extension reactions were loaded on a 6% urea polyacrylamide gel, with their expected size indicated on the right and molecular weight markers on the left. For gel source data, see Supplementary Fig. 1.