Extended Data Figure 5: Growth and fertility of offspring from in-vitro-generated oocytes. | Nature

Extended Data Figure 5: Growth and fertility of offspring from in-vitro-generated oocytes.

From: Reconstitution in vitro of the entire cycle of the mouse female germ line

Extended Data Figure 5

a, The number of 2-cell embryos and pups derived from BVSCH18 ESCs. b, Summary of eye colour and transgene of the pups (or adult mouse) derived from in-vitro-generated oocytes from BVSCH18 ESCs. c, Weights of placentas (left), newborn pups (middle) and development of the body weights (right) of offspring from the MII oocytes from BVSCH18 (closed circles) and the genetically matched wild-type mice (129X1/svjC57BL/6F1 × ICR) (closed circles) in 3 independent experiments (t-test, **P < 0.01, *P < 0.05) d, Combined bisulfite restriction analysis (COBRA) of DMRs of the imprinted genes (H19, Igf2r, Peg3 and Snrpn) in the 10 mice (1–10) derived from in-vitro-generated MII oocytes and the two wild-type mice (WT1 and WT2). PCR products were either digested (D) or undigested (U) with the respective enzyme. The digested and undigested fragments are indicated by black and white triangles, respectively. e, Bisulfite sequence analysis of DMRs of the imprinted genes in the two mice (1 and 2) from in-vitro-generated oocytes and the wild-type mouse (WT1). White and black circles represent unmethylated and methylated CpG sequences, respectively. f, A female (left) and a male (right) mouse from in-vitro-generated oocytes with full fertility. The table below the images shows the number of pups in a litter. g, The 6 mice derived from in-vitro-generated MII oocytes at 11 months after birth. For gel source data see Supplementary Fig. 1.

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