Extended Data Figure 5: Histological controls and closed-loop stimulation technique. | Nature

Extended Data Figure 5: Histological controls and closed-loop stimulation technique.

From: Prefrontal neuronal assemblies temporally control fear behaviour

Extended Data Figure 5

a, Representative micrograph used for dmPFC GFP expression assessment. b, Representative dmPFC PV interneurons with distinct dendritic arborizations were infected. Left: multipolar neuron with a round soma, corresponding to a putative basket cell. Right: interneuron with an ovoid soma at the layer 1–2 border, displaying asymmetric and tufted dendrites. Some branches extend towards the pia, bend and follow the pial surface. This neuron corresponds to the typical description of axo-axonic (chandelier) cells in mPFC. c, Top: representative raw (black trace) and filtered (grey trace, 3–6 Hz) dmPFC LFP recorded in PV-IRES-Cre mice during 4 Hz oscillations (top) or in CAMKII-Cre mice outside 4 Hz oscillations infected with ChR2 (bottom) upon light activation (30 ms square pulses; black line, pulse start; red bar, pulse length). d, e, Averaged LFP power spectra obtained in ChR2-infected mice and GFP controls during stimulated (coloured traces) and non-stimulated (grey traces) periods when the stimulation was applied in the ascending (ChR2, n = 7 mice; GFP, n = 7 mice) or descending (ChR2, n = 7 mice; GFP, n = 7 mice) phase. f, Averaged 3–6 Hz LFP power for ChR2 and GFP mice during stimulation in the ascending or descending phase of 4 Hz oscillations expressed as a percentage of no stimulation periods (ChR2, n = 7 mice; GFP, n = 7 mice; two-way ANOVA, factor 1: GFP versus ChR2, P = 0.848; factor 2: descending versus ascending, P = 0.879; F1 × F2, P = 0.618). g, h, Averaged LFP power spectra obtained in ArchT infected mice and GFP controls during stimulated (coloured traces) and non-stimulated (grey traces) periods when the stimulation was applied in the descending (ArchT, n = 5 mice; GFP, n = 7 mice) or ascending phase (ChR2, n = 5 mice; GFP, n = 4 mice). i, Averaged 3–6 Hz LFP power for ArchT and GFP mice during stimulation in the descending or ascending phase of 4 Hz oscillations expressed as a percentage of no stimulation periods (ArchT, n = 5 mice; GFP, n = 7/4 mice; two-way ANOVA, factor 1: GFP versus ChR2, P = 0.872; factor 2: descending versus ascending, P = 0.183; F1 × F2, P = 0.5). Error bars, mean ± s.e.m.

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