Extended Data Figure 3 : Processivity of BE1.
From: Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
The protospacer and PAM (blue) of a 60-mer DNA oligonucleotide containing eight consecutive Cs is shown at the top. The oligonucleotide (125 nM) was incubated with BE1 (2 μM) for 2 h at 37 °C. The DNA was isolated and analysed by high-throughput sequencing. Shown are the percent of total reads for the most frequent nine sequences observed. The vast majority of edited strands (>93%) have more than one C converted to T.
