Extended Data Figure 6 : Base editing persists over multiple cell divisions.
From: Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Cellular C to T conversion percentages by BE2 and BE3 are shown for HEK293 sites 3 and 4 in HEK293T cells before and after passaging the cells. HEK293T cells were nucleofected with plasmids expressing BE2 or BE3 and an sgRNA targeting HEK293 site 3 or 4. Three days after nucleofection, the cells were harvested and split in half. One half was subjected to high-throughput sequencing analysis, and the other half was allowed to propagate for approximately five cell divisions, then harvested and subjected to high-throughput sequencing analysis. Values and error bars reflect the mean and standard deviation of two biological experiments performed on different days.
