a, The sequence of full-length AtLURE1.2 accompanied by lysine/arginine residues (yellow highlight) mutated to glycines for AtLURE1.2(GGGG). Cysteine residues in the mature peptide are shown in red. b, c, Semi-in-vivo attraction assay using gelatine beads containing 5 μM His–AtLURE1.2(GGGG) (b) and wavy assay using 10 μM His–AtLURE1.2(GGGG) in the medium (c). The AtLURE1.2(GGGG) peptide showed no activity in these assays. The data are representative of 14 or 3 samples for b or c, respectively. Scale bars, 20 μm (b) and 200 μm (c).