Extended Data Figure 1: Co-existence of electrical and chemical synapses. | Nature

Extended Data Figure 1: Co-existence of electrical and chemical synapses.

From: Motor neurons control locomotor circuit function retrogradely via gap junctions

Extended Data Figure 1

a, Representative recording of a V2a interneuron and a motor neuron connected with mixed synapses (n = 11 from 11 zebrafish). EPSP amplitude induced in the motor neuron by stimulation of the V2a interneuron was reduced by blockers of ionotropic glutamate receptors (AP-5 and NBQX) leaving only an electrical event. b, Stimulation of the motor neuron also induced an electrical event in the V2a interneuron that was insensitive to the glutamate receptor blockers. c, Changes in the amplitude of the synaptic events in motor neurons and V2a interneurons induced by glutamate receptor blockers (*P < 0.05, two-tailed Student’s t-test, error bars denote s.e.m.). d, Representative recording of a V2a interneuron and a motor neuron (n = 12 from 12 zebrafish) in which blockade of chemical synaptic transmission with cadmium decreased the EPSP amplitude leaving only an electrical event. e, Cadmium did not affect the amplitude of the electrical events induced in the V2a interneuron by stimulation of the motor neuron. f, Effect of cadmium on the synaptic events in motor neurons and V2a interneurons (*P < 0.05, two-tailed Student’s t-test, error bars denote s.e.m). g, Representative recording of a V2a interneuron and a motor neuron connected with chemical synapses (n = 11 from 11 zebrafish). Blockade of ionotropic glutamate receptors completely abolished the V2a interneuron-induced EPSP in the motor neuron. h, In this pair, stimulation of the motor neuron did not induce any electrical event in the V2a interneuron. i, Effects of glutamate blockers and cadmium on the synaptic events induced in motor neurons and V2a interneurons (***P < 0.001, two-tailed Student’s t-test, error bars denote s.e.m.).

Back to article page