Extended Data Figure 7: Effects of wild-type and mutant Rmrp in T-cell differentiation. | Nature

Extended Data Figure 7: Effects of wild-type and mutant Rmrp in T-cell differentiation.

From: DDX5 and its associated lncRNA Rmrp modulate TH17 cell effector functions

Extended Data Figure 7

a, Il17a mRNA in cell lysates of in vitro polarized mouse TH17 cells at 96 h after transduction of control vector or wild-type Rmrp. Results are representative of two independent experiments. b, IFNγ production in polarized mouse TH1 cells at 96 h after transduction of control or Rmrp-encoding vector. Representative of two independent experiments. Each experiment was performed with two technical replicates. c, Comparison of human and mouse Rmrp sequences. Several mutations identified in CHH patients are highlighted. d, IL-17A production in polarized mouse TH17 cells at 96 h after transduction of wild-type or mutant Rmrp vectors. Representative of two independent experiments. e, Venn diagram depicting the number of distinct and overlapping genes regulated by RORγt, DDX5 and Rmrp in in vitro polarized TH17 cells. f, Expression of cytokine and Foxp3 mRNAs in T cells from wild-type or RmrpG270T/G270T mice cultured in vitro in TH17-, iTreg-, TH1- and TH2-polarizing conditions. Results are representative of two independent experiments. Each experiment was performed with two technical replicates. ***P < 0.001 (unpaired, t-test). g, ChIP-qPCR experiment using anti-RORγ/γt antibodies on chromatin of TH17 cells from wild-type or mutant mice cultured for 48 h in vitro. Each dot represents a different biological sample. Wild type, n = 2; RmrpG270T, n = 2. Results are representative of three separate independent experiments. Graphs show mean ± s.d. (unpaired, t-test).

Source data

Back to article page