a, Timeline of ASO treatment and behavioural tests. b, Two weeks after cessation of ASO treatment, MECP2-TG mice showed no rescue of hypoactivity, rearing behaviour or anxiety parameters in the open field test. c, No rescue was evident in any of the parameters of the elevated plus maze test at this early post-treatment stage. d, ASO treatment normalized performance in the rotarod test in MECP2-TG mice (asterisks indicate significance between MECP2-TG ASO and control-ASO groups). e, The impaired social behaviour in the 3-chamber test was not normalized in the ASO-treated group. No significant difference was found between any of the groups in the time spent investigating the inanimate object. f, No preference for the cups placed in the left or right chambers was found in the habituation phase of the 3-chamber test (n = 19 for wild-type group; n = 16 for transgenic group; n = 15 for TG-ASO group (for all behavioural tests)). Data were analysed by one-way ANOVA followed by Fisher’s LSD post hoc test, with the exception of the rotarod test that was analysed by two-way ANOVA repeated measures followed by Tukey HSD post hoc correction for multiple comparisons. Data are mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001.