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Widespread exploitation of the honeybee by early Neolithic farmers


The pressures on honeybee (Apis mellifera) populations, resulting from threats by modern pesticides, parasites, predators and diseases, have raised awareness of the economic importance and critical role this insect plays in agricultural societies across the globe. However, the association of humans with A. mellifera predates post-industrial-revolution agriculture, as evidenced by the widespread presence of ancient Egyptian bee iconography dating to the Old Kingdom (approximately 2400 bc)1. There are also indications of Stone Age people harvesting bee products; for example, honey hunting is interpreted from rock art2 in a prehistoric Holocene context and a beeswax find in a pre-agriculturalist site3. However, when and where the regular association of A. mellifera with agriculturalists emerged is unknown4. One of the major products of A. mellifera is beeswax, which is composed of a complex suite of lipids including n-alkanes, n-alkanoic acids and fatty acyl wax esters. The composition is highly constant as it is determined genetically through the insect’s biochemistry. Thus, the chemical ‘fingerprint’ of beeswax provides a reliable basis for detecting this commodity in organic residues preserved at archaeological sites, which we now use to trace the exploitation by humans of A. mellifera temporally and spatially. Here we present secure identifications of beeswax in lipid residues preserved in pottery vessels of Neolithic Old World farmers. The geographical range of bee product exploitation is traced in Neolithic Europe, the Near East and North Africa, providing the palaeoecological range of honeybees during prehistory. Temporally, we demonstrate that bee products were exploited continuously, and probably extensively in some regions, at least from the seventh millennium cal bc, likely fulfilling a variety of technological and cultural functions. The close association of A. mellifera with Neolithic farming communities dates to the early onset of agriculture and may provide evidence for the beginnings of a domestication process.

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Figure 1: High-temperature gas chromatography/mass spectrometry chromatograms of total lipid extract of a sherd from Çayönü Tepesi (6500–6000 cal bc) containing beeswax.
Figure 2: Partial gas chromatograms of total lipid extracts from Neolithic sherds from each geographical region.
Figure 3: Geographical distribution of prehistoric sites in the date range 7500 and 2000 cal bc yielding beeswax residues.
Figure 4: Regional distribution of beeswax residues in potsherd lipid extracts.

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  1. Crane, E. The World History of Beekeeping and Honey Hunting (Duckworth, 1999)

  2. Dams, M. & Dams, L. R. Spanish art rock depicting honey gathering during the Mesolithic. Nature 268, 228–230 (1977)

    Article  ADS  Google Scholar 

  3. d’Errico, F. et al. Early evidence of San material culture represented by organic artifacts from Border Cave, South Africa. Proc. Natl Acad. Sci. USA 109, 13214–13219 (2012)

    Article  ADS  Google Scholar 

  4. Larson, G. & Fuller, D. Q. The evolution of animal domestication. Annu. Rev. Ecol. Evol. Syst. 45, 115–136 (2014)

    Article  Google Scholar 

  5. Wallberg, A. et al. A worldwide survey of genome sequence variation provides insight into the evolutionary history of the honeybee Apis mellifera. Nature Genet. 46, 1081–1088 (2014)

    Article  CAS  Google Scholar 

  6. Ruttner, F. Biogeography and Taxonomy of Honeybees (Springer, 1987)

  7. Buckland, P. I. & Buckland, P. C. in Versions: BugsCEP v7.63; Bugsdata v8.01; BugsMCR v2.02; BugStats v1.22 (2006)

  8. Limbrey, S. in Archaeological Aspects of Woodland Ecology (eds Bell, M. & Limbrey, S. ) 279–286 (British Archaeological Reports, 1982)

  9. Wilson, E. O. The Insect Societies (Belknap Press of Harvard Univ. Press, 1971)

  10. Regert, M., Colinart, S., Degrand, L. & Decavallas, O. Chemical alteration and use of beeswax through time: accelerated ageing tests and analysis of archaeological samples from various environmental contexts. Archaeometry 43, 549–569 (2001)

    Article  CAS  Google Scholar 

  11. Mayyas, A. S., Al-Qudah, M. A., Douglas, K. A. & Al-Ajlouny, F. K. Beeswax preserved in archaeological ceramics: function and use. Annals of Faculty of Arts. Ain Shams University 40, 343–371 (2012)

    Google Scholar 

  12. Copley, M. S. et al. Dairying in antiquity. III. Evidence from absorbed lipid residues dating to the British Neolithic. J. Archaeol. Sci. 32, 523–546 (2005)

    Article  Google Scholar 

  13. Heron, C., Nemcek, N., Bonfield, K. M., Dixon, D. & Ottaway, B. S. The chemistry of Neolithic beeswax. Naturwissenschaften 81, 266–269 (1994)

    Article  ADS  CAS  Google Scholar 

  14. Decavallas, O. in Cooking up the Past: Food and Culinary Practices in the Neolithic and Bronze Age Aegean (eds Mee, C. & Renard, J. ) 148–157 (Oxford Books Limited, 2007)

  15. Evershed, R. P., Vaughan, S. J., Dudd, S. N. & Soles, J. S. Fuel for thought? Beeswax in lamps and conical cups from Late Minoan Crete. Antiquity 71, 979–985 (1997)

    Article  Google Scholar 

  16. Evershed, R. P., Dudd, S. N., Anderson-Stojanovic, V. R. & Gebhard, E. R. New chemical evidence for the use of combed ware pottery vessels as beehives in ancient Greece. J. Archaeol. Sci. 30, 1–12 (2003)

    Article  Google Scholar 

  17. Salque, M. et al. Earliest evidence for cheese making in the sixth millennium bc in northern Europe. Nature 493, 522–525 (2013)

    Article  ADS  CAS  Google Scholar 

  18. Aichholz, R. & Lorbeer, E. Investigation of combwax of honeybees with high-temperature gas chromatography and high-temperature gas chromatography–chemical ionization mass spectrometry: I. high-temperature gas chromatography. J. Chromatogr. A 855, 601–615 (1999)

    Article  CAS  Google Scholar 

  19. Garnier, N., Cren-Olivé, C., Rolando, C. & Regert, M. Characterization of archaeological beeswax by electron ionization and electrospray ionization mass spectrometry. Anal. Chem. 74, 4868–4877 (2002)

    Article  CAS  Google Scholar 

  20. Evershed, R. P. et al. Earliest date for milk use in the Near East and southeastern Europe linked to cattle herding. Nature 455, 528–531 (2008)

    Article  ADS  CAS  Google Scholar 

  21. Mellaart, J. Excavations at Çatal Hüyük, 1962: second preliminary report. Anatolian Studies 13, 43–103 (1963)

    Google Scholar 

  22. Regert, M., Dudd, S. N., Van Bergen, P. F., Pétrequin, P. & Evershed, R. P. Investigations of solvent extractable lipids and insoluble polymeric components: organic residues in Neolithic ceramic vessels from Chalain (Jura, France). British Archaeological Reports 939, 78–90 (2001)

    Google Scholar 

  23. Šoberl, L. et al. Neolithic and Eneolithic activities inferred from organic residue analysis of pottery from Mala Triglavca, Moverna vas and Ajdovska jama, Slovenia. Documenta Praehistorica 41, 149–179 (2014)

    Article  Google Scholar 

  24. Heron, C., Craig, O. E., Forster, M. & Stern, B. & Andersen, S. H. in Shell Middens in Atlantic Europe (eds Milner, N., Craig, O. E. & Bailey, G. N. ) Ch. 7, 78–85 (Oxbow Books, 2007)

    Google Scholar 

  25. Carreck, N. Are honey bees (Apis mellifera L.) native to the British Isles? J. Apic. Res. 47, 318–322 (2008)

    Article  Google Scholar 

  26. Cramp, L. J. E. et al. Immediate replacement of fishing with dairying by the earliest farmers of the northeast Atlantic archipelagos. Proc. R. Soc. Lond. B 281, 20132372 (2014)

    Article  Google Scholar 

  27. Cramp, L. J. E. et al. Neolithic dairy farming at the extreme of agriculture in northern Europe. Proc. R. Soc. Lond. B 281, 20140819 (2014)

    Article  Google Scholar 

  28. Outram, A. K. et al. The earliest horse harnessing and milking. Science 323, 1332–1335 (2009)

    Article  ADS  CAS  Google Scholar 

  29. Bartlein, P. J. et al. Pollen-based continental climate reconstructions at 6 and 21 ka: a global synthesis. Clim. Dyn. 37, 775–802 (2011)

    Article  Google Scholar 

  30. Abou-Shaara, H. F. The foraging behaviour of honey bees, Apis mellifera: a review. Vet. Med. (Praha) 59, 1–10 (2014)

    Article  Google Scholar 

  31. Bernardini, F. Beeswax as dental filling on a Neolithic human tooth. PLoS ONE 7, e44904 (2012)

    Article  ADS  CAS  Google Scholar 

  32. Akima, H. A method of bivariate interpolation and smooth surface fitting for irregularly distributed data points. ACM Trans. Math. Softw. 4, 148–159 (1978)

    Article  Google Scholar 

  33. Akima, H. Algorithm 761: scattered-data surface fitting that has the accuracy of a cubic polynomial. ACM Trans. Math. Softw. 22, 362–371 (1996)

    Article  Google Scholar 

  34. The R Core Team. R: a Language and Environment for Statistical Computing. (R Foundation for Statistical Computing, 2012)

Download references


We thank the UK Natural Environment Research Council for partial funding of the mass spectrometry facilities at Bristol (contract no. R8/H10/63; and English Heritage, European Research Council, Leverhulme Trust, Ministère de la Culture et de la Communication, Ministère de l’Enseignement Supérieur et de la Recherche (ACI Jeunes Chercheurs), Natural Environment Research Council, Région PACA, Royal Society and Wellcome Trust for funding.

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Authors and Affiliations



M.R.-S., M.R., R.P.E. and A.K.O. conceived and planned the project about beeswax in prehistory. M.R.-S., M.R. and R.P.E. wrote the paper. M.R.-S., M.R., L.J.E.C., O.D., J.D., S.Mil., S.Mir., M.P., J.S., L.S., H.L.W., M.Bart. and D.U.-K. undertook planning of regional lipid residue analyses projects, sampling, analytical work and data analysis. P.G. created Figure 4 and Supplementary Information section 3. All other authors either directed excavations or provided expertise in relation to pottery collections and essential insights into the study region and sites.

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Correspondence to Mélanie Roffet-Salque, Martine Regert or Richard P. Evershed.

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The authors declare no competing financial interests.

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Supplementary information

Supplementary Information

This file contains Supplementary Information sections 1-3. Section 1 contains a table showing Neolithic sites from which sherds were analysed with summary of results of lipid residue analyses. Mesolithic sites were added where available. Section 2 contains a table showing archaeological contexts for sherds containing beeswax. Section 3 contains statistical analysis of the lipid residue dataset. (PDF 305 kb)

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Roffet-Salque, M., Regert, M., Evershed, R. et al. Widespread exploitation of the honeybee by early Neolithic farmers. Nature 527, 226–230 (2015).

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