a, In vitro translated proteins were subjected to GST–LC3B pull-down. b, BJ ER:HRasV12 cells were analysed by immunoblotting. Uncropped blots are in Supplementary Figure 1. c, d, Colony formation analysis of BJ ER:HRasV12 cells. A590 nm, absorbance at 590 nm. e, Mid-life BJ fibroblasts stably expressing mCherry–GFP-tagged constructs were recorded for growth. Uncropped blots are in Supplementary Figure 1. f, Day 60, quantified for β-gal positivity. g, Day 101, quantified for cytoplasmic DAPI. Bars, mean ± s.e.m. (c, d), s.d. (f, g); n = 3 (c, d), n = 4 (f, g); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; NS, non-significant; one-way ANOVA coupled with Tukey’s post hoc test. h, Schematic illustration of autophagy degradation of nuclear lamina.